Ya se ha desarrollado los alcanos (hidrocarburos saturados) que forman parte de las naftas de mejor octanaje, por tecnología recombinante bacteriana, con Escherichia coli, en el 2013....


http://www.nature.com/nature/journal...ture12536.html

Microbial production of short-chain alkanes

Yong Jun Choi & Sang Yup Lee
AffiliationsContributionsCorresponding author
Nature (2013) doi:10.1038/nature12536
Received 11 March 2013 Accepted 08 August 2013 Published online 29 September 2013


Increasing concerns about limited fossil fuels and global environmental problems have focused attention on the need to develop sustainable biofuels from renewable resources. Although microbial production of diesel has been reported, production of another much in demand transport fuel, petrol (gasoline), has not yet been demonstrated. Here we report the development of platform Escherichia coli strains that are capable of producing short-chain alkanes (SCAs; petrol), free fatty acids (FFAs), fatty esters and fatty alcohols through the fatty acyl (acyl carrier protein (ACP)) to fatty acid to fatty acyl-CoA pathway. First, the β-oxidation pathway was blocked by deleting the fadE gene to prevent the degradation of fatty acyl-CoAs generated in vivo. To increase the formation of short-chain fatty acids suitable for subsequent conversion to SCAs in vivo, the activity of 3-oxoacyl-ACP synthase (FabH)1, which is inhibited by unsaturated fatty acyl-ACPs2, was enhanced to promote the initiation of fatty acid biosynthesis by deleting the fadR gene; deletion of the fadR gene prevents upregulation of the fabA and fabB genes responsible for unsaturated fatty acids biosynthesis3. A modified thioesterase4 was used to convert short-chain fatty acyl-ACPs to the corresponding FFAs, which were then converted to SCAs by the sequential reactions of E. coli fatty acyl-CoA synthetase, Clostridium acetobutylicum fatty acyl-CoA reductase and Arabidopsis thaliana fatty aldehyde decarbonylase. The final engineered strain produced up to 580.8 mg l−1 of SCAs consisting of nonane (327.8 mg l−1), dodecane (136.5 mg l−1), tridecane (64.8 mg l−1), 2-methyl-dodecane (42.8 mg l−1) and tetradecane (8.9 mg l−1), together with small amounts of other hydrocarbons. Furthermore, this platform strain could produce short-chain FFAs using a fadD-deleted strain, and short-chain fatty esters by introducing the Acinetobacter sp. ADP1 wax ester synthase (atfA)5 and the E. coli mutant alcohol dehydrogenase (adhEmut)


Para la fecha, hay varias investigaciones que dan más claridad de los mecanismos enzimáticos comprometidos en estas novedosas biosíntesis.

http://html.rhhz.net/zghxkb/20150408.htm


Naturalmente se producen por biosíntesis ciertos alcanos como son el metano, etano y otros que forman, por ej, los gases intestinales. Pero estos alcanos son gases. Lo interesante de estas investigaciones es que logran sintetizar alcanos de cadena corta pero que no son gases, sino líquidos, y como tal, son algunos de los que forman las naftas (seguramente, ramificados, porque de esa manera logran aumentar el P. Ebullición, a fin de lograr el estado líquido).

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